deadtm viability cytotoxicity assay kit Search Results


deadtm viability cytotoxicity assay kit  (U.S Everbright)
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U.S Everbright deadtm viability cytotoxicity assay kit
FIGURE 2 | SW induced cytoplasmic vacuolization and inhibited cell viability in RTECs (A,B) Phase-contrast images of RTECs following incubation with SW at the indicated concentrations for 24 h. Morphometric analysis of vacuoles was performed with three different areas and the percentage of SW-induced vacuolated cells was calculated (C,D) RTECs were treated with the indicated concentrations of SW for 24 h and cellular viability was measured using Live and <t>DeadTM</t> Viability/Cytotoxicity Assay Kit. Analyze the number of living (green spots) and dead cells (red spots) in three different areas of control and SW-treated cells and the ratios were calculated.
Deadtm Viability Cytotoxicity Assay Kit, supplied by U.S Everbright, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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deadtm viability cytotoxicity assay kit - by Bioz Stars, 2026-10
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FIGURE 2 | SW induced cytoplasmic vacuolization and inhibited cell viability in RTECs (A,B) Phase-contrast images of RTECs following incubation with SW at the indicated concentrations for 24 h. Morphometric analysis of vacuoles was performed with three different areas and the percentage of SW-induced vacuolated cells was calculated (C,D) RTECs were treated with the indicated concentrations of SW for 24 h and cellular viability was measured using Live and DeadTM Viability/Cytotoxicity Assay Kit. Analyze the number of living (green spots) and dead cells (red spots) in three different areas of control and SW-treated cells and the ratios were calculated.

Journal: Frontiers in pharmacology

Article Title: Swainsonine Triggers Paraptosis via ER Stress and MAPK Signaling Pathway in Rat Primary Renal Tubular Epithelial Cells.

doi: 10.3389/fphar.2021.715285

Figure Lengend Snippet: FIGURE 2 | SW induced cytoplasmic vacuolization and inhibited cell viability in RTECs (A,B) Phase-contrast images of RTECs following incubation with SW at the indicated concentrations for 24 h. Morphometric analysis of vacuoles was performed with three different areas and the percentage of SW-induced vacuolated cells was calculated (C,D) RTECs were treated with the indicated concentrations of SW for 24 h and cellular viability was measured using Live and DeadTM Viability/Cytotoxicity Assay Kit. Analyze the number of living (green spots) and dead cells (red spots) in three different areas of control and SW-treated cells and the ratios were calculated.

Article Snippet: Live and DeadTM Viability/Cytotoxicity Assay Kit was purchased from US Everbright® Inc. (Suzhou, China).

Techniques: Incubation, Cytotoxicity Assay, Control

FIGURE 8 | Effects of 4-PBA on SW-induced cytoplasmic vacuolization and cell death. RTECs were pretreated with 4-PBA at the indicated concentrations for 4 h and further treated with 400 μg/ml SW for 24 h (A,B) Western blotting was used to analyze the Bip protein level changes in the presence of 4-PBA. Three independent experiments were performed. *p < 0.05, **p < 0.01 (C,D) Inverted microscopic images of RTECs treated with SW (400 μg/ml) in the presence of 4-PBA. Morphometric analysis of vacuoles was performed with three different areas and the percentage of SW-induced vacuolated cells was calculated (E,F) Cellular viability was assessed using Live and DeadTM Viability/Cytotoxicity Assay Kit. Analyze the number of living (green spots) and dead cells (red spots) in three different areas of control and SW-treated cells and the ratios were calculated.

Journal: Frontiers in pharmacology

Article Title: Swainsonine Triggers Paraptosis via ER Stress and MAPK Signaling Pathway in Rat Primary Renal Tubular Epithelial Cells.

doi: 10.3389/fphar.2021.715285

Figure Lengend Snippet: FIGURE 8 | Effects of 4-PBA on SW-induced cytoplasmic vacuolization and cell death. RTECs were pretreated with 4-PBA at the indicated concentrations for 4 h and further treated with 400 μg/ml SW for 24 h (A,B) Western blotting was used to analyze the Bip protein level changes in the presence of 4-PBA. Three independent experiments were performed. *p < 0.05, **p < 0.01 (C,D) Inverted microscopic images of RTECs treated with SW (400 μg/ml) in the presence of 4-PBA. Morphometric analysis of vacuoles was performed with three different areas and the percentage of SW-induced vacuolated cells was calculated (E,F) Cellular viability was assessed using Live and DeadTM Viability/Cytotoxicity Assay Kit. Analyze the number of living (green spots) and dead cells (red spots) in three different areas of control and SW-treated cells and the ratios were calculated.

Article Snippet: Live and DeadTM Viability/Cytotoxicity Assay Kit was purchased from US Everbright® Inc. (Suzhou, China).

Techniques: Western Blot, Cytotoxicity Assay, Control